PCR mix consisted of mM dNTPs, μM of each primer, U/μl Prime Taq DNA Polymerase, 1× prime Taq Reaction Buffer, mM of MgCl 2, and 5 ng/μl template DNA. Cycler conditions were as follows: initial denaturation at 94 °C for 3 min; followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 54 °C (ITS) or 56 °C (gpd) for 30 s, and extension .
WhatsApp: +86 18203695377· In Pakistan, we generally use Margalla Crush, Sargodha Crush and Sakhi Sarwar Crush. Fig. 6: Coarse Aggregates. Any aggregate particle that passes sieve #4 sieve and retains of #200 sieve is termed as 'fine aggregate '. Fine aggregates (also termed as fillers) are added to fill up the voids created by the coarse aggregates. Sand is used as a filler material. In .
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WhatsApp: +86 18203695377B min. = A min. minus 2 x E max. B max. = A max. minus 2 x E min. Squeeze is measured from the bottom of the groove to the mating surface and includes the clearance gap. The following formula is used to determine the actual gland depth with tolerances: Max. Gland Depth = max. bore minus min. groove diameter, divided by 2. Min. Gland Depth = min ...
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WhatsApp: +86 18203695377Backfill is used to replace soil or dirt that was excavated from the ground. This can include a garden site for raised beds or material to place behind a retaining wall. Typically when calculating backfill, the material will not include any large rocks or forms of vegetation.
WhatsApp: +86 18203695377· Organic acids were added at concentrations of 1 mM and each counter ion ( Mg 2+, NH 4 + and PO 4 3−) was added at concentrations of 1 μM, 10 μM, 100 μM and 1 mM. The organic acids selected are known to be exuded by plant root systems (Jones 1998 ), and are mono (acetic acid), di (malic acid, oxalic acid) or trivalent (citric acid).
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WhatsApp: +86 18203695377Therefore, fineness modulus of aggregate = (cumulative % retained) / 100 = (275/100) = Fineness modulus of fine aggregate is It means the average value of aggregate is in between the 2 nd sieve and 3 rd sieve. It means the average aggregate size is in between to as shown in below figure. Values of Fineness Modulus of Sand
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